Review



ultracruz hardset mounting medium plus dapi  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology ultracruz hardset mounting medium plus dapi
    Amiloride promoted ErbB3 translocation from the nucleus to the cytoplasm and the plasma membrane in HSPC cells ( A ) Hormone-sensitive LNCaP cells were treated with varying concentrations of amiloride for 72 h before being lysed, fractionated and analyzed by immunoblot. ( B ) Immunofluorescence microscopy (IF) in LNCaP cells treated with DMSO or 75 µM or and probed with IF specific C-terminal ErbB3 antibodies or ( C ) 10µM or 25µM amiloride and probed with IF specific N-terminal ErbB3 antibodies for 72 h (scale bars = 30 μm). Note that vehicle treated LNCaP cells expressed nuclear ErbB3 (red) whereas amiloride-treated cells had significantly decreased ErbB3 expression in the nucleus (hollowed out). Location of nuclei are identified by blue <t>DAPI</t> staining. Plasma membrane localization of ErbB3 at cell-cell junction was also noted in amiloride-treated but not in vehicle treated cells. Note that both N- and C-terminal ErbB3 antibodies demonstrate lighter nuclear staining in amiloride-treated cells. ( D ) Cells were subjected to viability assays using the stated concentrations of amiloride. p-values are calculated with respect to DMSO
    Ultracruz Hardset Mounting Medium Plus Dapi, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ultracruz+hardset+mounting+medium+plus+dapi/pmc11671466-66-16-22?v=Santa+Cruz+Biotechnology
    Average 95 stars, based on 92 article reviews
    ultracruz hardset mounting medium plus dapi - by Bioz Stars, 2026-08
    95/100 stars

    Images

    1) Product Images from "Amiloride sensitizes prostate cancer cells to the reversible tyrosine kinase inhibitor lapatinib by modulating Erbb3 subcellular localization"

    Article Title: Amiloride sensitizes prostate cancer cells to the reversible tyrosine kinase inhibitor lapatinib by modulating Erbb3 subcellular localization

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-024-05540-5

    Amiloride promoted ErbB3 translocation from the nucleus to the cytoplasm and the plasma membrane in HSPC cells ( A ) Hormone-sensitive LNCaP cells were treated with varying concentrations of amiloride for 72 h before being lysed, fractionated and analyzed by immunoblot. ( B ) Immunofluorescence microscopy (IF) in LNCaP cells treated with DMSO or 75 µM or and probed with IF specific C-terminal ErbB3 antibodies or ( C ) 10µM or 25µM amiloride and probed with IF specific N-terminal ErbB3 antibodies for 72 h (scale bars = 30 μm). Note that vehicle treated LNCaP cells expressed nuclear ErbB3 (red) whereas amiloride-treated cells had significantly decreased ErbB3 expression in the nucleus (hollowed out). Location of nuclei are identified by blue DAPI staining. Plasma membrane localization of ErbB3 at cell-cell junction was also noted in amiloride-treated but not in vehicle treated cells. Note that both N- and C-terminal ErbB3 antibodies demonstrate lighter nuclear staining in amiloride-treated cells. ( D ) Cells were subjected to viability assays using the stated concentrations of amiloride. p-values are calculated with respect to DMSO
    Figure Legend Snippet: Amiloride promoted ErbB3 translocation from the nucleus to the cytoplasm and the plasma membrane in HSPC cells ( A ) Hormone-sensitive LNCaP cells were treated with varying concentrations of amiloride for 72 h before being lysed, fractionated and analyzed by immunoblot. ( B ) Immunofluorescence microscopy (IF) in LNCaP cells treated with DMSO or 75 µM or and probed with IF specific C-terminal ErbB3 antibodies or ( C ) 10µM or 25µM amiloride and probed with IF specific N-terminal ErbB3 antibodies for 72 h (scale bars = 30 μm). Note that vehicle treated LNCaP cells expressed nuclear ErbB3 (red) whereas amiloride-treated cells had significantly decreased ErbB3 expression in the nucleus (hollowed out). Location of nuclei are identified by blue DAPI staining. Plasma membrane localization of ErbB3 at cell-cell junction was also noted in amiloride-treated but not in vehicle treated cells. Note that both N- and C-terminal ErbB3 antibodies demonstrate lighter nuclear staining in amiloride-treated cells. ( D ) Cells were subjected to viability assays using the stated concentrations of amiloride. p-values are calculated with respect to DMSO

    Techniques Used: Translocation Assay, Clinical Proteomics, Membrane, Western Blot, Immunofluorescence, Microscopy, Expressing, Staining



    Similar Products

    ultracruz hardset mounting medium plus dapi  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology ultracruz hardset mounting medium plus dapi
    Amiloride promoted ErbB3 translocation from the nucleus to the cytoplasm and the plasma membrane in HSPC cells ( A ) Hormone-sensitive LNCaP cells were treated with varying concentrations of amiloride for 72 h before being lysed, fractionated and analyzed by immunoblot. ( B ) Immunofluorescence microscopy (IF) in LNCaP cells treated with DMSO or 75 µM or and probed with IF specific C-terminal ErbB3 antibodies or ( C ) 10µM or 25µM amiloride and probed with IF specific N-terminal ErbB3 antibodies for 72 h (scale bars = 30 μm). Note that vehicle treated LNCaP cells expressed nuclear ErbB3 (red) whereas amiloride-treated cells had significantly decreased ErbB3 expression in the nucleus (hollowed out). Location of nuclei are identified by blue <t>DAPI</t> staining. Plasma membrane localization of ErbB3 at cell-cell junction was also noted in amiloride-treated but not in vehicle treated cells. Note that both N- and C-terminal ErbB3 antibodies demonstrate lighter nuclear staining in amiloride-treated cells. ( D ) Cells were subjected to viability assays using the stated concentrations of amiloride. p-values are calculated with respect to DMSO
    Ultracruz Hardset Mounting Medium Plus Dapi, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ultracruz+hardset+mounting+medium+plus+dapi/pmc11671466-66-16-22?v=Santa+Cruz+Biotechnology
    Average 95 stars, based on 1 article reviews
    ultracruz hardset mounting medium plus dapi - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Amiloride promoted ErbB3 translocation from the nucleus to the cytoplasm and the plasma membrane in HSPC cells ( A ) Hormone-sensitive LNCaP cells were treated with varying concentrations of amiloride for 72 h before being lysed, fractionated and analyzed by immunoblot. ( B ) Immunofluorescence microscopy (IF) in LNCaP cells treated with DMSO or 75 µM or and probed with IF specific C-terminal ErbB3 antibodies or ( C ) 10µM or 25µM amiloride and probed with IF specific N-terminal ErbB3 antibodies for 72 h (scale bars = 30 μm). Note that vehicle treated LNCaP cells expressed nuclear ErbB3 (red) whereas amiloride-treated cells had significantly decreased ErbB3 expression in the nucleus (hollowed out). Location of nuclei are identified by blue DAPI staining. Plasma membrane localization of ErbB3 at cell-cell junction was also noted in amiloride-treated but not in vehicle treated cells. Note that both N- and C-terminal ErbB3 antibodies demonstrate lighter nuclear staining in amiloride-treated cells. ( D ) Cells were subjected to viability assays using the stated concentrations of amiloride. p-values are calculated with respect to DMSO

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Amiloride sensitizes prostate cancer cells to the reversible tyrosine kinase inhibitor lapatinib by modulating Erbb3 subcellular localization

    doi: 10.1007/s00018-024-05540-5

    Figure Lengend Snippet: Amiloride promoted ErbB3 translocation from the nucleus to the cytoplasm and the plasma membrane in HSPC cells ( A ) Hormone-sensitive LNCaP cells were treated with varying concentrations of amiloride for 72 h before being lysed, fractionated and analyzed by immunoblot. ( B ) Immunofluorescence microscopy (IF) in LNCaP cells treated with DMSO or 75 µM or and probed with IF specific C-terminal ErbB3 antibodies or ( C ) 10µM or 25µM amiloride and probed with IF specific N-terminal ErbB3 antibodies for 72 h (scale bars = 30 μm). Note that vehicle treated LNCaP cells expressed nuclear ErbB3 (red) whereas amiloride-treated cells had significantly decreased ErbB3 expression in the nucleus (hollowed out). Location of nuclei are identified by blue DAPI staining. Plasma membrane localization of ErbB3 at cell-cell junction was also noted in amiloride-treated but not in vehicle treated cells. Note that both N- and C-terminal ErbB3 antibodies demonstrate lighter nuclear staining in amiloride-treated cells. ( D ) Cells were subjected to viability assays using the stated concentrations of amiloride. p-values are calculated with respect to DMSO

    Article Snippet: After washing thrice with cold PBST, coverslips were inverted and mounted onto uncharged glass slides with UltraCruz Hardset Mounting Medium plus DAPI (SantaCruz BioTech, Dallas, TX).

    Techniques: Translocation Assay, Clinical Proteomics, Membrane, Western Blot, Immunofluorescence, Microscopy, Expressing, Staining